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Human Fas/TNFRSF6/CD95 Antibody

Catalog # MAB142 | R&D Systems, Inc. a Bio-Techne Brand
Clone DX2 was used by HLDA to establish CD designation
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MAB142
MAB142-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DX2

Product Summary for Human Fas/TNFRSF6/CD95 Antibody

Immunogen

L cells transfected with human Fas

Specificity

Detects human Fas in direct ELISAs and Western blots. Does not cross-react with rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rmDR3, rhHVEM, rmHVEM, rhLymphotoxin betaR, rmLymphotoxin betaR, rhTRAIL R1, rmTRAIL R1, rhTRAIL R2, rmTRAIL R2, rhTRAIL R3, or rhTRAIL R4,

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Fas/TNFRSF6/CD95 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Western Blot

1 µg/mL
Sample: Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS)
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB142 in the following applications:

Published Applications

Read 4 publications using MAB142 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes, and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney, and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

References

  1. Nagata, S. and P. Golstein (1995) Science 267:1449.
  2. Nagata, S. (1997) Cell 88:355.
  3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
  4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Gene Symbol

FAS

Product Documents for Human Fas/TNFRSF6/CD95 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Fas/TNFRSF6/CD95 Antibody

For research use only

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Reconstitution Calculator

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