Human Fas/TNFRSF6/CD95 Antibody

Name: Human Fas/TNFRSF6/CD95 Antibody
Brand: R&D Systems
SKU: MAB142
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB142

Citations (4)

(1)

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Clone DX2 was used by HLDA to establish CD designation

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Key Product Details

Species Reactivity:	Human
Immunogen:	L cells transfected with human Fas
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # DX2
Specificity:	Detects human Fas in direct ELISAs and Western blots. Does not cross-react with rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rmDR3, rhHVEM, rmHVEM, rhLymphotoxin beta R, rmLymphotoxin beta R, rhTRAIL R1, rmTRAIL R1, rhTRAIL R2, rmTRAIL R2, rhTRAIL R3, or rhTRAIL R4,

View Full Technical Details for Human Fas/TNFRSF6/CD95 Antibody

Applications for Human Fas/TNFRSF6/CD95 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes, and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney, and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

Item	Value
References	Nagata, S. and P. Golstein (1995) Science 267:1449. Nagata, S. (1997) Cell 88:355. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
Long Name	Fibroblast-associated
Entrez Gene IDs	355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names	APT1, APT1FASTM, Apo-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95, CD95 antigen, CD95ALPS1A, FAS1, FASLG receptor, Fas, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, TNFRSF6, TNFRSF6member 6, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6

Citations for Human Fas/TNFRSF6/CD95 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Fas/TNFRSF6/CD95 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Harris LK et al. (2006), Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism. Am. J. Pathol., 169(5):1863-74.
PMID: 17071607
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Chopin V et al. (2004), Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1). Exp. Cell Res., 298(2):560-73.
PMID: 15265702
Species: Human
Sample Types: Whole Cells
Applications: ICC

Phillips DC et al. (2003), Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells. Biochem. J., 375(0):567-79.
PMID: 12877656
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Savarese TM et al. (2002), Coexpression of oncostatin M and its receptors and evidence for STAT3 activation in human ovarian carcinomas. Cytokine, 17(6):324-34.
PMID: 12061840

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Customer Reviews for Human Fas/TNFRSF6/CD95 Antibody (1)

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Human Fas/TNFRSF6/CD95 Antibody

Name: Anonymous

Application: WB

Sample Tested: Lymphocytes

Species: Human

Verified Customer | Posted 11/15/2021

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Product Documents for Human Fas/TNFRSF6/CD95 Antibody

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Human Fas/TNFRSF6/CD95 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Fas/TNFRSF6/CD95 Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Fas/TNFRSF6/CD95

Citations for Human Fas/TNFRSF6/CD95 Antibody (4)

Customer Reviews for Human Fas/TNFRSF6/CD95 Antibody (1)

Customer Images

Human Fas/TNFRSF6/CD95 Antibody

Product Documents for Human Fas/TNFRSF6/CD95 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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