FAM20B was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human FAM20B Monoclonal Antibody (Catalog # MAB84271) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human FAM20B Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Reviewed Applications

Read 1 review rated 5 using MAB84271 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM20B

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

References

Nalbant, D. et al. (2005) BMC Genomics 6:11.
Koike, T. et al. (2009) Biochem. J. 421:157.
Vogel, P. et al. (2012) Vet. Pathol. 49:998.
Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Family with Sequence Similarity 20, Member B

Alternate Names

Glycosaminoglycan Xylosylkinase, gxk1, XYLK, Xylose Kinase

Entrez Gene IDs

9917 (Human); 215015 (Mouse); 304885 (Rat)

Gene Symbol

FAM20B

UniProt

O75063

Additional FAM20B Products

Product Documents for Human FAM20B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FAM20B Antibody

For research use only

Citations
Reviews

Human FAM20B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB84271

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FAM20B Antibody

FAM20B in Human Brain (Cortex).