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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Avian - Chicken, Bovine, Camelid, Canine, Elephant, Equine, Feline, Hamster, Hamster - Cricetulus (Chinese Hamster), Insect - Drosophila, Plant - Nicotiana benthamiana, Primate - Pongo (Orangutan), Rhinoceros

Applications

Validated:

Western Blot, Immunoaffinity Purification

Cited:

Western Blot, ELISA Capture, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # AE7A5

Product Specifications

Immunogen

Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588

Specificity

Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Erythropoietin/EPO Antibody

Application
Recommended Usage

Immunoaffinity Purification

This antibody can be used with optimal binding of human EPO at pH 7-9 (1-3).

Western Blot

1 µg/mL
Sample: Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 286-EP)

Reviewed Applications

Read 1 review rated 5 using MAB2871 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

UniProt

Additional Erythropoietin/EPO Products

Product Documents for Human Erythropoietin/EPO Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Erythropoietin/EPO Antibody

For research use only

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