Human Erbin Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7866T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Erbin
Lys496-Thr687
Accession # Q96RT1
Lys496-Thr687
Accession # Q96RT1
Specificity
Detects human Erbin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse ErbB2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Erbin Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Erbin
ErbB2IP (ErbB2 Interacting Protein/Erbin; also Densin-180-like protein and LAP2) is both a nuclear and cytoplasmic 175-180 kDa member of the LAP (LRR And PDZ) family of proteins. It is widely expressed, and found in cell types as diverse as intestinal columnar epithelium, neurons, keratinocytes and skeletal plus cardiac muscle cells. Functionally, ErbB2IP interacts with multiple proteins through a PZD-mediated interaction. Partners include ErbB2, delta-catenin, PSD-95 and ARVCF. The ErbB2 interaction localizes this receptor to the basolateral membrane of epithelium, while the delta-catenin interaction impacts dendritic arborization, possibly through involvement with type 1 or 2 catenin(s) and the F-actin cytoskeleton. Finally, ErbB2IP/Erbin is suggested to play a role in maintaining the G1 phase of the cell cycle. Human ErbB2IP is 1412 amino acids (aa) in length. It contains 17 LRRs (Leu-rich repeats) (aa 23-413) followed by one PDZ domain (aa 1321-1410). There are more than 10 utilized Tyr, and 15 utilized Ser/Thr phosphorylation sites. A series of deletions over aa 1211-1377 generate at least six known splice variants. All show a deletion of aa 1212-1252, and at least one additional 10-41 aa deletion C-terminal to the common deletion. Over aa 496-687, human and mouse ErbB2IP share 88% aa sequence identity.
Long Name
ErbB2-interacting Protein
Alternate Names
Densin-180-like protein, ERBB2IP
Gene Symbol
ERBIN
UniProt
Additional Erbin Products
Product Specific Notices for Human Erbin Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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