Detects human EphA10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human EphA1, A2, A5, A6, B2, B3, B4, B6, recombinant mouse EphA3, A4, or A8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human EphA10 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Reviewed Applications

Read 2 reviews rated 5 using MAB5188 in the following applications:

Immunohistochemistry (1 Review)
Immunohistochemistry-Paraffin (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA10

EphA10 is a member of the Eph family of receptor tyrosine kinases that are activated by binding members of the Ephrin ligand family. EphA10 preferentially binds Ephrin-A3, -A4 and -A5. EphA10 mRNA expression appears limited to the testis, unlike most Ephs that are expressed in developing and adult neural tissue. Human EphA10 extracellular domain (ECD) shares 95% and 88% amino acid identity with mouse and rat EphA10, respectively. Of three alternately spliced isoforms, one is truncated within the ECD and another within the cytoplasmic domain.

Long Name

Eph Receptor A10

Alternate Names

EC 2.7.10.1, EPH receptor A10, EphA10s protein, ephrin type-A receptor 10, FLJ16103, FLJ33655, MGC43817

Entrez Gene IDs

284656 (Human); 298528 (Rat)

Gene Symbol

EPHA10

UniProt

Q5JZY3 (Human)

Additional EphA10 Products

Product Documents for Human EphA10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human EphA10 Antibody

For research use only

Citations
Reviews

Human EphA10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5188

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity