Human Endostatin Antibody

Name: Human Endostatin Antibody
Brand: R&D Systems
SKU: AF1098
Price: 157 USD

Catalog # AF1098 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Endostatin Antibody

Immunogen

E. coli-derived recombinant human Endostatin
His1572-Ser1753
Accession # P39060

Specificity

Detects human Endostatin in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse Endostatin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Endostatin Antibody

Endostatin in Human Kidney.

Endostatin was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Endostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1098) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Applications for Human Endostatin Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Human Endostatin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endostatin

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Alternate Names

COL18A1

Entrez Gene IDs

80781 (Human); 12822 (Mouse)

Gene Symbol

COL18A1

UniProt

P39060

Product Documents for Human Endostatin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Endostatin Antibody

For research use only

Citations for Human Endostatin Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Endostatin Antibody include:

Species: Human
Sample Types: Cell Fractions
Applications: Western Blot

S Sharma et al. (2021-12-22), E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects JCI Insight, 2021-16(24).
PMID: 34935642
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

M Holsti et al. (2018-04-12), Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue Eur J Vasc Endovasc Surg, 2018-00(0).
PMID: 29656960
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Baruah P et al. (2015-07-14), Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers. Br J Cancer, 2015-0113(4):653-9.
PMID: 26171937
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Ohlund D et al. (2008-06-15), Expression pattern and circulating levels of endostatin in patients with pancreas cancer. Int. J. Cancer, 2008-0122(12):2805-10.
PMID: 18360823
Species: Porcine
Sample Types: Cell Lysates
Applications: Western Blot

Bartsch G et al. (2007-11-19), Combined antiangiogenic therapy is superior to single inhibitors in a model of renal cell carcinoma. J. Urol., 2007-1179(1):326-32.
PMID: 18006013

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