Human EBI3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6456A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 607201
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
Arg21-Lys229 (predicted)
Accession # Q14213
Arg21-Lys229 (predicted)
Accession # Q14213
Specificity
Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human EBI3 APC-conjugated Antibody
Detection of IL‑27/IL‑35 EBI3 Subunit in KG‑1 Human Cell Line by Flow Cytometry.
KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-27/IL-35 EBI3 Subunit APC-conjugated Monoclonal Antibody (Catalog # IC6456A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human EBI3 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: KG‑1 human acute myelogenous leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: KG‑1 human acute myelogenous leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EBI3
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-Induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.
Long Name
Epstein-Barr virus induced gene 3
Alternate Names
EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3
Gene Symbol
EBI3
UniProt
Additional EBI3 Products
Product Documents for Human EBI3 APC-conjugated Antibody
Product Specific Notices for Human EBI3 APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...