Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human E-Selectin/P-Selectin (CD62E/P) Antibody

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells.

E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human E-Selectin/P-Selectin (CD62E/P) Antibody

Application

Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA-010)

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Reviewed Applications

Read 4 reviews rated 3.3 using BBA1 in the following applications:

Immunohistochemistry (2 Reviews)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names

Eselectin, PSelectin

Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products

Product Documents for Human E-Selectin/P-Selectin (CD62E/P) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human E-Selectin/P-Selectin (CD62E/P) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human E-Selectin/P-Selectin (CD62E/P) Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA1

Key Product Details

Species Reactivity

Validated:

Cited:

Applications