DLX5 was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Sheep Anti-Human DLX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6710) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DLX5 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into osteocytes

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLX5

DLX5 (distal-less homeobox 5) is a 33-35 kDa member of the distal-less homeobox family of transcription factors. It is expressed in embryonic mesenchyme where it promotes cartilage and bone formation, and in nervous tissue, where it promotes interneuron development. Human DLX5 is 289 amino acids (aa) in length. It contains one DNA-binding homeobox domain (aa 137-196), and two phosphorylation sites at Ser34 and Ser217 that, when utilized, increases transcriptional activity. DLX5 likely forms homodimers, and is known to heterodimerize with MSX-2. Over aa 216-289, human DLX5 shares 99% aa identity with mouse DLX5.

Long Name

Distal-less Homeobox 5

Alternate Names

distal-less homeo box 5, distal-less homeobox 5, homeobox protein DLX-5

Entrez Gene IDs

1749 (Human); 13395 (Mouse); 25431 (Rat)

Gene Symbol

DLX5

UniProt

P56178 (Human)

Additional DLX5 Products

Product Documents for Human DLX5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DLX5 Antibody

For research use only

Citations
Reviews

Human DLX5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6710

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all DLX5 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DLX5 Antibody

DLX5 in Human Mesenchymal Stem Cells.