Human DLL1 Antibody

Name: Human DLL1 Antibody
Brand: R&D Systems
SKU: AF1818

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1818

Citations (2)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human DLL1 Ser22-Gly540 Accession # AAG09716
Applications:	CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects recombinant human DLL1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human DLL4 and recombinant mouse DLL4 is observed.

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Applications for Human DLL1 Antibody

	Recommended Concentration	Sample
Flow Cytometry	1 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human DLL1 Antibody

	Flow Cytometry Detection of DLL1 in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Goat Anti-Human DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1818, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

Item	Value
References	Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. USA 104:15454. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Citations for Human DLL1 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human DLL1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Lemke J et al. (2014), Selective CDK9 inhibition overcomes TRAIL resistance by concomitant suppression of cFlip and Mcl-1. Cell Death Differ, 21(3):491-502.
PMID: 24362439
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

Stahl M et al. (2008), Roles of Pofut1 and O-fucose in mammalian Notch signaling. J. Biol. Chem., 283(20):13638-51.
PMID: 18347015

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Product Documents for Human DLL1 Antibody

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Human DLL1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human DLL1 Antibody

Scientific Data Examples for Human DLL1 Antibody

Flow Cytometry