Human DC-SIGNR/CD299 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB162AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Adhesion Blockade, Flow Cytometry, CyTOF-ready
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 120604
Product Specifications
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Specificity
Detects human DC‑SIGNR/CD299.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Adhesion Blockade
Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: DC-SIGNR/CD299
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
References
- Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
- Pohlmann, S. et al. (2003) J. Virol. 77:4070.
- Simmons, L.G. et al. (2003) J. Virol. 77:1337.
- Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
- Feinberg, H. et al. (2001) Science 294:2163.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
Alternate Names
CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN
Entrez Gene IDs
10332 (Human)
Gene Symbol
CLEC4M
UniProt
Additional DC-SIGNR/CD299 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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