Detects human Cystatin D in Western blots. In this format, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C and rhCystatin SN is observed, less than 5% cross-reactivity with rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin A, rhCystatin B and rhCystatin E/M is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cystatin D Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cystatin D (Catalog # 1202-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

References

Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.

Alternate Names

CST5, CSTD

Entrez Gene IDs

1473 (Human)

Gene Symbol

CST5

UniProt

CAA49838

Additional Cystatin D Products

Product Documents for Human Cystatin D Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Cystatin D Biotinylated Antibody

For research use only

Citations
Reviews

Human Cystatin D Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1202

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity