Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cystatins A, B, C, E/M, S, SA, SN, rhKininogen, rhFetuins A, B, or rhHPRG is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human Cystatin D Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cystatin D (Catalog # 1202-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cystatin D (Catalog # 1202-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human Cystatin D (1.3 µg/mL, Catalog # 1202-PI) inhibition of Papain (0.1 mg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 mM, Catalog # ES009). The Neutralization Dose (ND₅₀) is typically 2.8 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

References

Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.

Alternate Names

CST5, CSTD

Entrez Gene IDs

1473 (Human)

Gene Symbol

CST5

UniProt

P28325

Additional Cystatin D Products

Product Documents for Human Cystatin D Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cystatin D Antibody

For research use only

Citations
Reviews

Human Cystatin D Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1202

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity