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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
Arg41-Glu331 (Ser79Leu)
Accession # Q92496

Specificity

Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) CFHR1, rhCFHR2, and rhCFHR5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Complement Factor H-related 4/CFHR4

FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.

Alternate Names

CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4

Entrez Gene IDs

10877 (Human)

Gene Symbol

CFHR4

UniProt

Additional Complement Factor H-related 4/CFHR4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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