HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP (A) vs irrelevant transfectant (B) were stained with Mouse Anti-Human Complement Component C3aR APC-conjugated Monoclonal Antibody (Catalog # FAB5995A). View our protocol for Staining Membrane-associated Proteins.

Detection of Complement Component C3a R in Human Whole Blood Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human Complement Component C3a R APC-conjugated Monoclonal Antibody (Catalog # FAB5995A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Complement Component C3aR APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP or Human whole blood monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is a 70-80 kDa G-protein coupled 7-transmembrane sulfated glycoprotein that belongs to the G-protein coupled receptor 1 family of proteins. It is expressed inducibly or constitutively by platelets, eosinophils, monocytes, astrocytes, CD4⁺ T cells, dendritic cells, NK cells, mast cells and basophils, neutrophils, adipocytes, osteoclasts, osteoblasts macrophages, vascular endothelial cells, bronchial epithelium and smooth muscle. Engagement of C3a R by 77 kDa C3a has a number of effects that are context-dependent. C3a action on adipocytes facilitates glucose and fatty acid uptake, while in bone, C3a R (plus C5aR) drive the generation of Th1 and Th17 cells. Perhaps its best action is that of a mediator of anaphylaxis. On mast cells it induces histamine release, visceral smooth muscle contraction and vasodilation. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Alternate Names

AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Gene Symbol

C3AR1

UniProt

Q16581

Additional Complement Component C3aR Products

Product Documents for Human Complement Component C3aR APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Complement Component C3aR APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Complement Component C3aR APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5995A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Complement Component C3aR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Complement Component C3aR APC-conjugated Antibody

Detection of Complement Component C3aR in HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP vs irrelevant transfectant by Flow Cytometry