Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 269104

View all Complement Component C1r Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
Ser18-Asp705
Accession # NP_001724

Specificity

Detects human C1r in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) C1s or rhC1q R1 is observed. In Western blots, ths antibody recognizes rhC1r and the A/heavy chain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Complement Component C1r Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C1r (Catalog # 1807-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Component C1r (Catalog # 1807-SE) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1r

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain.

References

  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
  2. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
  3. Journet A. and M. Tosi (1986) Biochem. J. 240:783.

Alternate Names

C1r

Entrez Gene IDs

715 (Human)

Gene Symbol

C1R

UniProt

NP_001724

Additional Complement Component C1r Products

Product Documents for Human Complement Component C1r Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Component C1r Antibody

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.