Human CMG-2/ANTXR2 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2940A

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FAB2940A
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all CMG-2/ANTXR2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CMG‑2/ANTXR2 isoform 1
Gln34-Asn317
Accession # P58335

Specificity

Detects human CMG‑2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse CMG-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CMG-2/ANTXR2 APC-conjugated Antibody

Detection of CMG-2/ANTXR2 antibody in Human Whole Blood Monocytes antibody by Flow Cytometry.

Detection of CMG‑2/ANTXR2 in Human Whole Blood Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Goat Anti-Human CMG-2/ANTXR2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2940A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CMG-2/ANTXR2 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human whole blood monocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed Anthrax Toxin Receptor (ATR) family protein (1‑3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoform 4 shows a 12 aa insertion in the cytoplasmic region, isoform 2 shows a 103 aa deletion in the ECD, and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1‑5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore, and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence identity with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa sequence identity with ATR/TEM8.

References

  1. Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  4. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  5. Santelli, E. et al. (2004) Nature 430:905.
  6. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  7. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  8. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  9. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  10. Wei, W. et al. (2006) Cell 124:1141.
  11. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

Long Name

Capillary Morphogenesis Protein 2

Alternate Names

ANTXR2, cI-35, CMG2, ISH, JHS

Entrez Gene IDs

118429 (Human); 71914 (Mouse)

Gene Symbol

ANTXR2

UniProt

P58335

Additional CMG-2/ANTXR2 Products

Product Documents for Human CMG-2/ANTXR2 APC-conjugated Antibody

Product Datasheets

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Product Specific Notices for Human CMG-2/ANTXR2 APC-conjugated Antibody

For research use only

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