Claudin-4 was detected in immersion fixed PC-3 human prostate cancer cell line (positive staining) and HLDM-2 human Hodgkin's lymphoma cell line (negative staining) using Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB42191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Claudin-4 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Alternate Names

Claudin4, CLDN4, CPE-R, CPETR1, WBSCR8

Entrez Gene IDs

1364 (Human); 12740 (Mouse); 304407 (Rat)

Gene Symbol

CLDN4

UniProt

NP_001296

Additional Claudin-4 Products

Product Documents for Human Claudin-4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Claudin-4 Antibody

For research use only

Citations
Reviews

Human Claudin-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB42191

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Claudin-4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Claudin-4 Antibody

Claudin‑4 in PC-3 Human Cell Line.