Detects human CILP‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 N-terminal peptide is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human CILP-1 C-Terminal Fragment Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CILP-1 C-Terminal Fragment

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CILP-1

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725‑1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.

Long Name

Cartilage Intermediate Layer Protein 1

Alternate Names

CILP, CILP1

Entrez Gene IDs

8483 (Human); 214425 (Mouse); 315761 (Rat)

Gene Symbol

CILP

UniProt

O75339

Additional CILP-1 Products

Product Documents for Human CILP-1 C-Terminal Fragment Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CILP-1 C-Terminal Fragment Antibody

For research use only

Citations
Reviews

Human CILP-1 C-Terminal Fragment Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4477

Key Product Details

Species Reactivity

Validated:

Cited:

Applications