Human CEACAM-8/CD66b APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4246A

Recombinant Monoclonal Antibody.
Discontinued Product
FAB4246A has been discontinued. View all CEACAM-8/CD66b products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 913542

View all CEACAM-8/CD66b Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997

Specificity

Stains human CEACAM‑8/CD66b transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CEACAM-8/CD66b APC-conjugated Antibody

Detection of CEACAM-8/CD66b antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-8/CD66b APC-conjugated Monoclonal Antibody (Catalog # FAB4246A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CEACAM-8/CD66b antibody in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP antibody by Flow Cytometry.

Detection of CEACAM-8/CD66b in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line either (A) transfected with human CEACAM-8/CD66b or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-8/CD66b APC-conjugated Monoclonal Antibody (Catalog # FAB4246A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CEACAM-8/CD66b APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes and HEK293 human embryonic kidney cell line transfected with human CEACAM-8/CD66b and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-8/CD66b

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8) also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Gene Symbol

CEACAM8

UniProt

P31997

Additional CEACAM-8/CD66b Products

Product Documents for Human CEACAM-8/CD66b APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM-8/CD66b APC-conjugated Antibody

For research use only

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