Human CEACAM-8/CD66b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4246
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CEACAM‑8/CD66b
Gln35-His141
Accession # P31997
Gln35-His141
Accession # P31997
Specificity
Detects human CEACAM‑8/CD66b in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-6, and rhCEACAM-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CEACAM-8/CD66b Antibody
Detection of CEACAM-8/CD66b in Human Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-8/CD66b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).Applications for Human CEACAM-8/CD66b Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human CEACAM-8/CD66b
Sample: Recombinant Human CEACAM-8/CD66b
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-8/CD66b
CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Alternate Names
CD66b, CD67, CEACAM8, CGM6, NCA-95
Gene Symbol
CEACAM8
UniProt
Additional CEACAM-8/CD66b Products
Product Documents for Human CEACAM-8/CD66b Antibody
Product Specific Notices for Human CEACAM-8/CD66b Antibody
For research use only
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