Human CD5L Antibody

Name: Human CD5L Antibody
Brand: R&D Systems
SKU: AF2797
Rating: 4 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2797

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(4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CD5L Ser20-Gly347 Accession # O43866
Applications:	Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human CD5L in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant mouse CD5L and less than 1% cross-reactivity with recombinant human CD5 is observed.

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Applications for Human CD5L Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CD5L (Catalog # 2797-CL)
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD5L Antibody

	Immunohistochemistry CD5L in Human Spleen. CD5L was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CD5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2797) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD5L

CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4⁺CD8⁺ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).

Item	Value
References	Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1. Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5. Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39. Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151. Gebe, J.A. et al. (2000) Immunol. 99:78. Haruta, I. et al. (2001) J. Biol. Chem. 276:22910. Kuwata, K. et al. (2003) Am. J. Pathol. 162:837. Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813. Arai, S. et al. (2005) Cell Metab. 1:201. Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086. Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413. Tissot, J.D. et al. (2002) Electrophoresis 23:1203.
Long Name	CD5 Antigen-like
Entrez Gene IDs	922 (Human); 11801 (Mouse)
Alternate Names	AIM, API6, CD5 antigen-like, CD5 antigen-like (scavenger receptor cysteine rich family), CD5 molecule-like, CD5L, CT-2, IgM-associated peptide, PRO229, SP-alpha, Spalpha, apoptosis inhibitor 6

Citations for Human CD5L Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD5L Antibody include:

Species: Human
Sample Types: Serum
Applications: ELISA Development

Gray J et al. (2009), A proteomic strategy to identify novel serum biomarkers for liver cirrhosis and hepatocellular cancer in individuals with fatty liver disease. BMC Cancer, 9(0):271.
PMID: 19656391

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Customer Reviews for Human CD5L Antibody (4)

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Human CD5L Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 01/14/2021
Human CD5L Antibody

Name: Anonymous

Application: Microarrays

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 11/07/2018
Human CD5L Antibody

Name: Anonymous

Application: ELISA

Sample Tested: Plasma,Serum

Species: Human and Mouse

Verified Customer | Posted 11/07/2018
Human CD5L Antibody

Name: Anonymous

Application: MiAr

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 11/02/2018

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Human CD5L Antibody

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Applications for Human CD5L Antibody

Scientific Data Examples for Human CD5L Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD5L

Citations for Human CD5L Antibody (1)

Customer Reviews for Human CD5L Antibody (4)

Customer Images

Human CD5L Antibody

Human CD5L Antibody

Human CD5L Antibody

Human CD5L Antibody

Product Documents for Human CD5L Antibody

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