Detects human CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD39L3 is observed, and less than 2% cross-reactivity with recombinant human (rh) CD39L1 and rhCD39 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD39L3/ENTPD3 Antibody

CD39L3/ENTPD3 in Human Salivary Gland.

CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human salivary gland using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CD39L3/ENTPD3 in Human Pancreas.

CD39L3/ENTPD3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4400) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CD39L3/ENTPD3 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human salivary gland and human pancreas

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD39L3/ENTPD3 (Catalog # 4400-EN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L3/ENTPD3

Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3), encoded by the ENTPD3 gene and also known as CD39L3, is an integral membrane protein with an extracellular active site (1). Recombinant human NTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-3 hydrolyzes the beta- and gamma-phosphate residues of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nucleotides, NTPDase-3 plays a role in the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues (3).

References

Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 3

Alternate Names

ATPDase, CD39L3, ENTPD3, HB6, NTPDase-3

Entrez Gene IDs

956 (Human); 215446 (Mouse); 316077 (Rat)

Gene Symbol

ENTPD3

UniProt

O75355

Additional CD39L3/ENTPD3 Products

Product Documents for Human CD39L3/ENTPD3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD39L3/ENTPD3 Antibody

For research use only

Citations
Reviews

Human CD39L3/ENTPD3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4400

Key Product Details

Species Reactivity

Validated:

Cited:

Applications