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Human CD36/SR‑B3 Antibody

Catalog # MAB1955 | R&D Systems, Inc. a Bio-Techne Brand
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MAB1955
MAB1955-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 255619

Product Summary for Human CD36/SR‑B3 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36/SR-B3
Gly30-Asn439
Accession # P16671

Specificity

Detects human CD36 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) CD36 is observed. In Western blots, no cross-reactivity with rmCD36 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human CD36/SR‑B3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CD36/SR-B3 Fc Chimera (Catalog # 1955-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using MAB1955 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fA beta), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References

  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
  3. Husemann, J. et al. (2002) Glia 40:195.
  4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
  5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.

Long Name

Scavenger Receptor Class B, Member 3

Alternate Names

CD36, Collagen R, FAT, GPIIIb, GPIV, SCARB3, SR-B3, SRB3, Thrombospondin R

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Gene Symbol

CD36

UniProt

Additional CD36/SR-B3 Products

Product Documents for Human CD36/SR‑B3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD36/SR‑B3 Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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