Human CD25/IL-2R alpha Alexa Fluor® 647-conjugated Antibody

Name: Human CD25/IL-2R alpha Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: FAB623R

R&D Systems, part of Bio-Techne | Catalog # FAB623R

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 RÎ± Glu22-Cys213 Accession # P01589
Applications:	ELISA Capture, ELISA Detection, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Specificity:	Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha. In sandwich immunoassays, this antibody does not cross-react or interfere

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Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Item	Value
Long Name	Interleukin 2 Receptor alpha
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names	CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL-2Ra, IL2-RA, IL2R, IL2R alpha, IL2RA, TAC antigen, TCGFR, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55

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