Human CD1c/BDCA-1 Alexa Fluor® 750-conjugated Antibody

Name: Human CD1c/BDCA-1 Alexa Fluor® 750-conjugated Antibody
Brand: R&D Systems
SKU: AF5910S

R&D Systems, part of Bio-Techne | Catalog # AF5910S

Submit a review

Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCAâ€‘1 Ala19-Met302 (Phe300Ser) Accession # P29017
Applications:	Western Blot
Label:	Alexa Fluor 750 (Excitation=749 nm, Emission=775 nm)
Specificity:	Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.

View Full Technical Details for Human CD1c/BDCA-1 Alexa Fluor® 750-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD1c/BDCA-1

CD1c (Cluster of differentiation antigen 1c) is a 316 amino acid glycoprotein with a predicted molecular weight of 43 kDa. CD1c is a member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta ₂‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta ₂‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

Item	Value
Entrez Gene IDs	911 (Human)
Alternate Names	BDCA-1, BDCA1, CD1, CD1A, CD1c, CD1c antigen, CD1c molecule, R7, T-cell surface glycoprotein CD1c, c polypeptide, cortical thymocyte antigen CD1C, differentiation antigen CD1-alpha-3

Customer Reviews

There are currently no reviews for this product. Be the first to review Human CD1c/BDCA-1 Alexa Fluor® 750-conjugated Antibody and earn rewards!

Have you used Human CD1c/BDCA-1 Alexa Fluor® 750-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Product Documents

Product Datasheets

Download Product Datasheets