Human Cathepsin H Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7516G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Cathepsin H
Ala23-Val335
Accession # CAA34734
Ala23-Val335
Accession # CAA34734
Specificity
Detects human Cathepsin H in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse Cathepsin H is observed, and less than 1% cross-reactivity with recombinant human Cathepsin L2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Cathepsin H Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Cathepsin H
Cathepsin H (CTSH; alos CPSB) is a 28-30 kDa ubiquitously expressed member of the peptidase C1 family of cysteine lysosomal enzymes. Depending upon the isoform, it may either be secreted or retained in the lysosomes. Although this family of enzymes plays a key role in general intracellular molecule degradation, CTSH is also known to participate in other activities. Such activities include the activation of progranzyme B, the late stage processing of pulmonary surfactant protein B, and the cleavage, in endosomes, of TLR3, allowing for subsequent TLR signaling. Human preproCTSH is 335 amino acids (aa) in length. It contains one 22 aa signal peptide, a 75 aa prosequence (aa 23-97), an intervening "mini fragment" (aa 98-105), a second prosequence (aa 106-115), and a 220 aa C-terminal mature region that could be further cleaved after Asn292 into two peptide fragments. CTSH is self-activating. The 40-42 kDa proprecursor (aa 23-335) is cleaved thrice to initially generate a 30 kDa intermediate form, followed by a 28 kDa mature form that is covalently attached to the aforementioned "mini fragment". Further processing at the C-terminus may create a smaller 24-25 kDa mature segment. CTSH is unusual in that it possess both aminopeptidase and endopeptidase activity. While endopeptidase activity is intrinsic to the mature region, aminopeptidase activity requires the presence of a covalently-linked "mini fragment". There is one splice variant that shows a deletion of aa 11-22. Although this occurs within the signal sequence, the molecule is secreted and shows high enzymatic activity. Over aa 23-335, human CTSH shares 84% aa sequence identity with mouse CTSH.
Additional Cathepsin H Products
Product Specific Notices for Human Cathepsin H Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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