Detects human Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse (rm) CPB1 is observed and less than 5% cross-reactivity with recombinant human (rh) CPA1, rhCPA2, and rmCPA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carboxypeptidase B1/CPB1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase B1/
CPB1 (Catalog # 2897-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase B1/CPB1 (Catalog # 2897-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis (2). The deduced amino acid sequence of human CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 110), and a mature chain (residues 111 to 417). The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).

References

Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 831, Academic Press, San Diego.
Yamamoto, K.K. et al. (1992) J. Biol. Chem. 267:2575.

Alternate Names

CPB1, PASP, PCPB

Entrez Gene IDs

1360 (Human); 76703 (Mouse)

Gene Symbol

CPB1

UniProt

P15086

Additional Carboxypeptidase B1/CPB1 Products

Product Documents for Human Carboxypeptidase B1/CPB1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Carboxypeptidase B1/CPB1 Antibody

For research use only

Citations
Reviews

Human Carboxypeptidase B1/CPB1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2897

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Carboxypeptidase B1/CPB1 Antibodies »

Product Specifications

Immunogen

Specificity