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Human Carbonic Anhydrase XII/CA12 Biotinylated Antibody

Bio-Techne includes R&D Systems | Catalog # BAF2190

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BAF2190

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Carbonic Anhydrase XII/CA12 Biotinylated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12
Ala25-Gln291
Accession # O43570

Specificity

Detects human Carbonic Anhydrase XII/CA12 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse CA12 is observed and less than 1% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8 and 10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Carbonic Anhydrase XII/CA12 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase XII (Catalog # 2190-CA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XII/CA12

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
  3. Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
  4. Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
  5. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

Alternate Names

CA12

Entrez Gene IDs

771 (Human); 76459 (Mouse)

Gene Symbol

CA12

UniProt

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Human Carbonic Anhydrase XII/CA12 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Carbonic Anhydrase XII/CA12 Biotinylated Antibody

For research use only

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