Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

Carbonic Anhydrase VA/VB (CA5A/5B) in Human Liver.

Carbonic Anhydrase VA/VB (CA5A/5B) was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Carbonic Anhydrase VA/ VB (CA5A/5B) Monoclonal Antibody (Catalog # MAB5388) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VA/VB (CA5A/5B)

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.

Alternate Names

CA5;CA5AD;Carbonic anhydrase 5A, mitochondrial;CAV;CAVA;GS1-21A4.1

Entrez Gene IDs

763 (Human); 54233 (Rat)

Gene Symbol

CA5A

UniProt

P35218

Additional Carbonic Anhydrase VA/VB (CA5A/5B) Products

Product Documents for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

For research use only

Citations
Reviews

Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5388

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Carbonic Anhydrase VA/VB (CA5A/5B) Antibodies »

Product Specifications

Immunogen

Specificity