Human/Canine CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1699
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Human
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alphaM
Phe17-Asn1105
Accession # NP_001139280
Phe17-Asn1105
Accession # NP_001139280
Specificity
Detects human CD11b/Integrin alphaM in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Integrin alphaM and recombinant human Integrin alphaE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Canine CD11b/Integrin alpha M Antibody
Detection of CD11b/Integrin alphaM in Canine PBMCs by Flow Cytometry.
Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine CD11b/Integrin aM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Detection of CD11b/Integrin alpha M in PBMC monocytes cells by Flow Cytometry.
PBMC monocytes cells were stained with Goat Anti-Human/Canine CD11b/Integrin alpha M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Canine CD11b/Integrin alpha M Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells and canine peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells and canine peripheral blood mononuclear cells (PBMCs)
Western Blot
0.1 µg/mL
Sample: Recombinant Human CD11b/Integrin alphaM
Sample: Recombinant Human CD11b/Integrin alphaM
Reviewed Applications
Read 1 review rated 5 using AF1699 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
UniProt
Additional CD11b/Integrin alpha M Products
Product Documents for Human/Canine CD11b/Integrin alpha M Antibody
Product Specific Notices for Human/Canine CD11b/Integrin alpha M Antibody
For research use only
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