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Human BMP-2/BMP-4 Biotinylated Antibody

Bio-Techne includes R&D Systems | Catalog # BAF355

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BAF355

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human BMP-2/BMP-4 Biotinylated Antibody

Immunogen

E. coli-derived recombinant human BMP‑2
Ala284-Arg396
Accession # P12643

Specificity

Detects human BMP‑2/BMP‑4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human BMP-2/BMP-4 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat neonatal dorsal root ganglia cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMP-2 (Catalog # 355-BM)
Recombinant Human BMP-4 (Catalog # 314-BP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using BAF355 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

References

  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Wozney, J. et al. (1988) Science 242:1528.
  4. Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269. 
  5. Sebald, W. et al. (2004) Biol. Chem. 385:697.
  6. De Luca, F. et al. (2001) Endocrinology 142:430.
  7. Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
  8. Ryoo, H.-M. et al. (2006) Gene 366:51.
  9. Kramer, J. et al. (2000) Mech. Dev. 92:193.
  10. Li, X. et al. (2008) Atherosclerosis January 5 epub.
  11. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  12. Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
  13. Kano, Y. et al. (2005) Endocrinology 146:5332.
  14. Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
  15. Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

Long Name

Bone Morphogenetic Protein 2/4

Alternate Names

BDA2, BMP-2, BMP2A, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

Additional BMP-2/BMP-4 Products

Product Documents for Human BMP-2/BMP-4 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BMP-2/BMP-4 Biotinylated Antibody

For research use only

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