Human beta-Actin Antibody

Name: Human beta-Actin Antibody
Brand: R&D Systems
SKU: MAB8969

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB8969

Citations (5)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Peptide containing a sequence at the N-terminus of human beta ‑Actin Accession # P60709
Applications:	Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 937210
Specificity:	Detects human beta ‑Actin in direct ELISAs.

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Applications for Human beta-Actin Antibody

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human beta-Actin Antibody

	Immunocytochemistry beta ‑Actin in HeLa Human Cell Line. beta -Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta -Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Item	Value
Entrez Gene IDs	60 (Human); 11461 (Mouse); 81822 (Rat)
Alternate Names	ACTB, B-Actin, BRWS1, Beta Actin, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1, actin, beta, actin, cytoplasmic 1, beta cytoskeletal actin, beta-Actin, betaActin

Citations for Human beta-Actin Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human beta-Actin Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

K Kwa?niak et al. (2022), LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells Cells, 11(3).
PMID: 35159188
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

P Shen et al. (2018), The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway Mol Med Rep, 18(3):3332-3340.
PMID: 30066927
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

B Liu et al. (2018), Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF?? Mol Med Rep, 18(2):1987-1994.
PMID: 29956764
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Y Mao et al. (2018), Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells Oncol Lett, 15(5):6599-6603.
PMID: 29731857
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot

Bing Liu et al. (2018), Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??. Molecular Medicine Reports, 0(0):1791-3004.
PMID: 29956764

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Showing 1 - 5 of 5 citations Showing All

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