Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody

Catalog # MAB85601 | R&D Systems, Inc. a Bio-Techne Brand
Human B3GAT1 MAb (Clone 1021236)
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MAB85601-100
MAB85601-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1021236

Product Summary for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody

Immunogen

Chinese Hamster Ovary cell line CHO-derived human beta‑1,3‑Glucuronyltransferase 1/B3GAT1
His25-Ile334
Accession # Q9P2W7

Specificity

Detects human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody

beta‑1,3‑Glucuronyltransferase 1/B3GAT1 antibody in SH‑SY5Y Human Cell Line by Immunocytochemistry (ICC).

beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in SH‑SY5Y Human Cell Line.

beta‑1,3‑Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line (positive) and A549 human lung carcinoma cell line (negative control) using Mouse Anti-Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our Fluorescent ICC Staining of Cells on Coverslips Protocol.

Applications for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta1-3Gal beta1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

  1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
  2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
  3. Kakuda, S. et al. (2005) Glycobiology 2:203.
  4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
  5. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Alternate Names

CD57, GLCATP, GLCUATP, HNK1, LEU7

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Gene Symbol

B3GAT1

UniProt

Q9P2W7

Product Documents

Certificate of Analysis

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Product Specific Notices for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody

For research use only

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