Human Aurora A Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3295
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aurora A
Met1-Glu131
Accession # O14965
Met1-Glu131
Accession # O14965
Specificity
Detects human Aurora A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Aurora B and rhAurora C is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Aurora A Biotinylated Antibody
Aurora A in HeLa Human Cell Line.
Aurora A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Aurora A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3295) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to mitotic spindles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Aurora A Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Aurora A
Sample: Recombinant Human Aurora A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora A
Alternate Names
AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7
Gene Symbol
AURKA
UniProt
Additional Aurora A Products
Product Documents for Human Aurora A Biotinylated Antibody
Product Specific Notices for Human Aurora A Biotinylated Antibody
For research use only
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