Human ATG10 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5464AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects endogenous human ATG10 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ATG10 Alexa Fluor™ Plus 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: ATG10
ATG10 (autophagy-related protein 10; also APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates non‑covalently with an ATG16 multimer to generate an autophagosome. Human ATG10 is 220 amino acids in length and contains an active site at Cys166 that forms a thiol ester bond with the C-terminal Gly of ATG12. There are multiple isoform variants. One shows a deletion of aa 37‑72, a second contains an alternate start site at Met43 that is accompanied by a 39 aa substitution for aa 152‑220, and a third shows a 53 aa substitution for aa 73‑220. Over amino acids 1‑190, human ATG10 shares 79% aa identity with mouse ATG10.
Long Name
Autophagy Related 10 Homolog
Alternate Names
APG10, APG10L, PP12616
Gene Symbol
ATG10
UniProt
Additional ATG10 Products
Product Specific Notices for Human ATG10 Alexa Fluor™ Plus 405-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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