Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ASAH2/N‑acylsphingosine Amidohydrolase-2
Thr15-Ile761
Accession # AAF86240

Specificity

Detects human ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3557-AH), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ASAH2/N-acylsphingosine Amidohydrolase-2 (Catalog # 3557-AH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The human ASAH2 gene encodes N-acylsphingosine amidohydrolase-2, also known as neutral or non-lysosomal ceramidase. ASAH2 is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). An N-terminally truncated form of human ASAH2 has been shown to localize to mitochondria (4). A major physiological function of ASAH2 is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

References

  1. Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
  2. Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
  3. Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
  4. El Bawab, S. et al. (2000) J. Biol. Chem. 275:21508.

Long Name

Neutral Ceramidase

Alternate Names

Nacylsphingosine Amidohydrolase2

Entrez Gene IDs

56624 (Human); 54447 (Mouse); 114104 (Rat)

Gene Symbol

ASAH2

UniProt

AAF86240

Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products

Product Documents for Human ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

For research use only

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