Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein B/ApoB in Human Liver.

Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Apolipoprotein B/ApoB by Simple Western^TM.

Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 312 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human Apolipoprotein B/ApoB Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

5 µg/mL
Sample: Human plasma

Western Blot

1 µg/mL
Sample: Human plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B/ApoB

ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Gene Symbol

APOB

UniProt

NP_000375

Additional Apolipoprotein B/ApoB Products

Product Documents for Human Apolipoprotein B/ApoB Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Apolipoprotein B/ApoB Antibody

For research use only

Citations
Reviews

Human Apolipoprotein B/ApoB Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3556

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Apolipoprotein B/ApoB Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Apolipoprotein B/ApoB Antibody

Detection of Human Apolipoprotein B/ApoB by Western Blot.