Immunoblot analyses of ERAP2 using LCL protein extract.Two LCLs of each ERAP2 genotype (AA, AB, and BB) were tested for protein using primary antibodies specific to: A, ERAP2 (goat polyclonal); B, ERAP2 (mouse polyclonal); and C, beta-actin (see Materials and Methods). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1001157), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Aminopeptidase LRAP/ERAP2 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN)

Reviewed Applications

Read 1 review rated 4 using AF3830 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase LRAP/ERAP2

Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).

References

Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.
Tanioka, T. et al. (2005) FEBS J. 272:916.
Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.

Long Name

Leukocyte-derived Arginine Aminopeptidase

Alternate Names

ERAP2

Entrez Gene IDs

64167 (Human)

Gene Symbol

ERAP2

UniProt

Q6P179

Additional Aminopeptidase LRAP/ERAP2 Products

Product Documents for Human Aminopeptidase LRAP/ERAP2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Aminopeptidase LRAP/ERAP2 Antibody

For research use only

Citations
Reviews

Human Aminopeptidase LRAP/ERAP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3830

Key Product Details

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Applications

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

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Host

Isotype

Scientific Data Images for Human Aminopeptidase LRAP/ERAP2 Antibody

Detection of Human Aminopeptidase LRAP/ERAP2 by Western Blot