25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Aminoacylase/ACY1 (Catalog # 2900-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.

References

Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
Maceyka, M. et al. (2004) FEBS Lett. 568:30.

Alternate Names

ACY1

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Gene Symbol

ACY1

UniProt

Q03154

Additional Aminoacylase/ACY1 Products

Product Documents for Human Aminoacylase/ACY1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Aminoacylase/ACY1 Antibody

For research use only

Citations
Reviews

Human Aminoacylase/ACY1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2900

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Aminoacylase/ACY1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Human Aminoacylase/ACY1 Antibody

Immunoprecipitation