Human alpha-Methylacyl-CoA Racemase/AMACR Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7508U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human α‑Methylacyl‑CoA Racemase/AMACR
Met1-Leu382
Accession # Q9UHK6
Met1-Leu382
Accession # Q9UHK6
Specificity
Detects human alpha‑Methylacyl‑CoA Racemase/AMACR in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: alpha-Methylacyl-CoA Racemase/AMACR
AMACR (Alpha-MethylAcyl-CoA Racemase; also 2-methylacyl racemase) is a 43-46 kDa member of the CaiB/BaiF CoA-transferase family of enzymes. It is widely expressed, being found in fibroblasts, hepatocytes, plus tumorigenic prostatic and colonic epithelium. Within these cells, it is localized to peroxisomes (organelles that participate in the breakdown fatty acids into 2-carbon blocks) and occasionally mitochondria, and appears to racemize 2-methyl-branched fatty acids. This ability is necessary for the degradation of branched fatty acids such as C19 dietary pristanic acid. Pristanic acid occurs in both an S- and R-methylated stereoisomer, but can only be initially degraded in the S- isomeric form. AMACR converts the R- to the S-isoform, initiating fatty acid processing. Human AMACR(-IA) is 382 amino acids (aa) in length. It contains an N-terminal mitochondrial targeting sequence (aa 22-85) that overlaps the enzymatic region (aa 53-231), and a C-terminal peroxisomal targeting motif (aa 379-382). There are multiple potential splice variants. Over aa 132-382, there are three aa substitutions, one that is 66 aa in length (AMACR‑IB), a second that is 147 aa in length (AMACR-IIB), and a third that is 98 aa in length. Over aa 249-382, there are two aa substitutions, one that is 13 aa in length (AMACR‑IIAs), and another that is 41 aa in length (AMACR-IIA). There is also a sixth potential splice variant that shows a 16 aa substitution for aa 378-382. Full-length human AMACR(-IA) shares 77% aa sequence identity with mouse AMACR.
Alternate Names
alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE
Gene Symbol
AMACR
UniProt
Additional alpha-Methylacyl-CoA Racemase/AMACR Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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