Human Alkaline Phosphatase/
ALPL Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1448
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry, Immunocytochemistry, Cell Selection, MACS
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # B4-78
Product Specifications
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Alkaline Phosphatase/ALPL Biotinylated Antibody
Alkaline Phosphatase/ALPL in Human Osteoblasts.
Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Alkaline Phosphatase/ALPL Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed differentiated human osteoblasts
Sample: Immersion fixed differentiated human osteoblasts
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine
Sample: Immersion fixed paraffin-embedded sections of human intestine
Reviewed Applications
Read 1 review rated 4 using BAM1448 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
References
- Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
- Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
- Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Human Alkaline Phosphatase/ALPL Biotinylated Antibody
Product Specific Notices for Human Alkaline Phosphatase/ALPL Biotinylated Antibody
For research use only
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