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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Aldo-keto Reductase 1C4/AKR1C4
Met1-Tyr323
Accession # NP_001809

Specificity

Detects human Aldo-keto Reductase 1C4/AKR1C4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AKR-1C1 and rhAKR-1C3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Aldo-keto Reductase 1C4/AKR1C4

AKR1C4 (Aldo-Keto Reductase family 1 member C4; also 3-alpha HSD1/3-alpha HSD type 1, Chlordecone/kepone reductase/CDR and DD4) is a monomeric, 36-38 kDa member of the four gene 3-alpha HSD family, aldo-keto reductase superfamily of enzymes. AKRs catalyse the reduction of aldehydes and ketones into alcohols through a NADPH-dependent process. The resulting alcohols become the target of subsequent conjugation reactions. AKR1C4 in particular is noted for its action on dihydroxytestosterone, converting it to a less active 3 alpha-diol. In addition, it also converts progesterone into 20 alpha-hydroxyprogesterone, a molecule that both induces GnRH release, and blocks the generation of (deoxy)cortisol from 17 alpha-hydroxyprogesterone. Finally, AKR1C4 likely is involved in the tetoxification of xenobiotics and drugs. AKR1C4 expression is essentially limited to hepatocytes and likely breast epithelium. Human AKR1C4 is 323 amino acids (aa) in length. Full-length human AKR1C4 shares 83% and 76% aa sequence identity with human AKR1C1 and mouse AKR1C4, respectively.

Long Name

Aldo-keto Reductase Family 1, Member C4

Alternate Names

3-alpha-HSD, AKR1C4, AldoketoReductase 1C4, CDR, CHDR, DD4, HAKRA

Entrez Gene IDs

1109 (Human)

Gene Symbol

AKR1C4

UniProt

Additional Aldo-keto Reductase 1C4/AKR1C4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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