Human ADAM8 aa 498-653 Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB10311AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 143338
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain
Specificity
Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ADAM8
ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).
References
- Yoshiyama, K. et al. (1997) Genomics 41:56.
- Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
- Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
- Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
- Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
- King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.
Long Name
A Disintegrin and Metalloprotease-like Domain 8
Alternate Names
CD156a, MS2
Entrez Gene IDs
101 (Human)
Gene Symbol
ADAM8
UniProt
Additional ADAM8 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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