Human 20S Proteasome Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB95541
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 863425
Product Specifications
Immunogen
Human 20S Proteasome purified from human erthrocytes
Specificity
Detects human 20S Proteasome in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human 20S Proteasome Antibody
20S Proteasome in Human Lung Cancer Tissue.
20S Proteasome was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB95541) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human 20S Proteasome Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 20S Proteasome
The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.
Additional 20S Proteasome Products
Product Documents for Human 20S Proteasome Antibody
Product Specific Notices for Human 20S Proteasome Antibody
For research use only
Loading...
Loading...
Loading...
Loading...