HspB11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88332

Novus Biologicals, part of Bio-Techne
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NBP1-88332
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HspB11 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IDLCLSSEGSEVILATSSDEKHPPENIIDGNPETFWTTTGMFPQEFIICFHKHVRIERLVIQSYFVQT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HspB11 Antibody - BSA Free

HspB11 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: HspB11 Antibody - BSA Free [NBP1-88332]

Immunocytochemistry/ Immunofluorescence: HspB11 Antibody - BSA Free [NBP1-88332]

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: HspB11 Antibody [NBP1-88332]

Immunohistochemistry-Paraffin: HspB11 Antibody [NBP1-88332]

Immunohistochemistry-Paraffin: HspB11 Antibody [NBP1-88332] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
HspB11 Antibody - BSA Free Western Blot: HspB11 Antibody - BSA Free [NBP1-88332]

Western Blot: HspB11 Antibody - BSA Free [NBP1-88332]

Analysis in control (vector only transfected HEK293T lysate) and HSPB11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for HspB11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HspB11

Alternate Names

C1orf41, heat shock protein beta-11, heat shock protein family B (small), member 11, Hspb11, HSPCO34, IFT25, intraflagellar transport 25 homolog, Placental protein 25, PP25chromosome 1 open reading frame 41

Gene Symbol

IFT25

Additional HspB11 Products

Product Documents for HspB11 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HspB11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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