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HSP90 Antibody (16F1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97506

Novus Biologicals, part of Bio-Techne
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NBP1-97506-0.2mg
NBP1-97506-0.05mg
NBP1-97506-0.025mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Fish, Guinea Pig, Invertebrate, Mammal, Opossum, Plant, Primate, Rabbit, Sheep, Xenopus

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 16F1

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Native human Hsp90.

Reactivity Notes

Opossum, Mussel, Beluga

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for HSP90 Antibody (16F1) - BSA Free

Western Blot: HSP90 Antibody (16F1) [NBP1-97506]

Western Blot: HSP90 Antibody (16F1) [NBP1-97506]

Western Blot: HSP90 Antibody (16F1) [NBP1-97506] - Lane 1: MW marker, Lane 2: HSP90 native protein, Lane 3: HSP90b recombinant protein, Lane 4: HSP90alpha recombinant protein, Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).
Immunohistochemistry-Paraffin: HSP90 Antibody (16F1) [NBP1-97506]

Immunohistochemistry-Paraffin: HSP90 Antibody (16F1) [NBP1-97506]

Immunohistochemistry-Paraffin: HSP90 Antibody (16F1) [NBP1-97506] - Analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10ug/ml.
HSP90 Antibody (16F1) - BSA Free

Western Blot: HSP90 Antibody (16F1) - BSA Free [NBP1-97506] -

Ixazomib increases heat-shock proteins (HSPs) in mouse Schwann cellsImmortalized mouse Schwann cells treated with either 10 μg/mL Ixazomib (Ixa) or dimethyl sulfoxide (DMSO) for 24 h were subjected to isobaric tags for relative and absolute quantitation-mass spectrometry (iTRAQ-MS) proteomics.(A) Overview of the 66 significantly changed proteins (false discovery rate [FDR]-corrected p < 0.05) in four Ixazomib (Ixa) groups as compared with three DMSO groups. The heatmap represents the log2 (fold change [FC]) of the differentially expressed proteins in each group. The color key from blue to red represents the log2 (FC) from low to high. See Tables S3A and S3B for details.(B) Enrichment analysis of differentially expressed proteins. The vertical axis represents the pathway category and the horizontal axis represents the enrichment score [–log10 (P)] of the pathway. See Table S3C for details.(C) Volcano plot representing log10 (FDR) as a function of log2 (FC). The proteins identified with an FDR-value <0.05 and >1.0-fold are highlighted in red (up-regulated) or blue (down-regulated), with the dotted lines representing the boundary for identification.(D) Immunoblotting analyses using antibodies against poly-ubiquitin (polyUb), HSP40, HSP70, HSP90, and proteasome modulator 9 (PSMD9).(E) Quantification of relative protein amounts in (D). beta-Actin was used for internal control. Unpaired t test. Data are represented As means +/- SEM. ns, not significant, ∗p < 0.05, ∗∗p < 0.01, N = 3 for untreated (DMSO), and 4 for Ixazomib. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S258900422301074X), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HSP90 Antibody (16F1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP90

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (16F1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP90 Antibody (16F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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