HSP60 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77396
![Flow (Intracellular): HSP60 Antibody - BSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Flow-Intracellular-NBP1-77396-img0010.jpg "Flow (Intracellular): HSP60 Antibody - BSA Free [NBP1-77396]")
![Western Blot: HSP60 AntibodyBSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Western-Blot-NBP1-77396-img0007.jpg "Western Blot: HSP60 AntibodyBSA Free [NBP1-77396]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Hamster, Primate
Cited:
Human, Primate - Macaca mulatta (Rhesus Macaque)
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 300-360). [Swiss-Prot P10809]
Localization
Mitochondrion matrix.
Marker
Mitochondria Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSP60 Antibody - BSA Free
Western Blot: HSP60 AntibodyBSA Free [NBP1-77396]
Western Blot: HSP60 Antibody [NBP1-77396] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7![Immunocytochemistry/ Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0004.jpg "Immunocytochemistry/ Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77396]")
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77396]
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77396] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).![Immunohistochemistry: HSP60 Antibody - BSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Immunohistochemistry-NBP1-77396-img0006.jpg "Immunohistochemistry: HSP60 Antibody - BSA Free [NBP1-77396]")
Immunohistochemistry: HSP60 Antibody - BSA Free [NBP1-77396]
Immunohistochemistry: HSP60 Antibody [NBP1-77396] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.![Immunocytochemistry/ Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77396-img0005.jpg "Immunocytochemistry/ Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77396]")
![Simple Western: HSP60 AntibodyBSA Free [NBP1-77396]](https://resources.bio-techne.com/images/products/HSP60-Antibody-Simple-Western-NBP1-77396-img0009.jpg "Simple Western: HSP60 AntibodyBSA Free [NBP1-77396]")
Applications for HSP60 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Simple Western
1:5000
Western Blot
0.5 ug/mL
Application Notes
In Western blot, a band is seen at ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HSP60
Long Name
Heat Shock Protein 60
Alternate Names
cpn60, GROEL, HSPD1, SPG13
Gene Symbol
HSPD1
UniProt
Additional HSP60 Products
Product Documents for HSP60 Antibody - BSA Free
Product Specific Notices for HSP60 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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