HSP60 Antibody (23GB2680)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51147

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NBP3-51147-100ul
NBP3-51147-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2680

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP60 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HSP60 (Uniprot: P10809)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP60 Antibody (23GB2680)

HSP60 Antibody (23GB2680)

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (23GB2680)[NBP3-51147] -

HSP60 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Hsp60 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
HSP60 Antibody (23GB2680)

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (23GB2680)[NBP3-51147] -

Immunocytochemical staining of HT-1080 cells with HSP60 antibody (1:1,000). Nuclei were stained blue with DAPI; HSP60 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
HSP60 Antibody (23GB2680)

Flow Cytometry: HSP60 Antibody (23GB2680)[NBP3-51147] -

Validation of HSP60 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HSP60 antibody (1:2,000) and analyzed using BD flow cytometer.
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Applications for HSP60 Antibody (23GB2680)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP60

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, arthrosclerosis and chlamydial disease.

Long Name

Heat Shock Protein 60

Alternate Names

cpn60, GROEL, HSPD1, SPG13

Gene Symbol

HSPD1

Additional HSP60 Products

Product Documents for HSP60 Antibody (23GB2680)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP60 Antibody (23GB2680)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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