HSP27 Antibody (HSPB1/774) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48002R

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-44975

Alexa Fluor 350

NBP2-48002AF350

Alexa Fluor 405

NBP2-48002AF405

Alexa Fluor 488

NBP2-48002AF488

Alexa Fluor 532

NBP2-48002AF532

Alexa Fluor 594

NBP2-48002AF594

Alexa Fluor 647

NBP2-48002AF647

Alexa Fluor 700

NBP2-48002AF700

Alexa Fluor 750

NBP2-48002AF750

Alexa Fluor Plus 405

NBP2-48002AFP405

Alexa Fluor Plus 488

NBP2-48002AFP488

Alexa Fluor Plus 555

NBP2-48002AFP555

Alexa Fluor Plus 594

NBP2-48002AFP594

Alexa Fluor Plus 647

NBP2-48002AFP647

Alexa Fluor Plus 680

NBP2-48002AFP680

Allophycocyanin

NBP2-48002APC

Biotin

NBP2-48002B

CoraFluor 1

NBP2-48002CL1

DyLight 350

NBP2-48002UV

DyLight 405

NBP2-48002V

DyLight 488

NBP2-48002G

DyLight 594

NBP2-48002DL594

DyLight 650

NBP2-48002C

DyLight 680

NBP2-48002FR

DyLight 755

NBP2-48002IR

FITC

NBP2-48002F

HRP

NBP2-48002H

Janelia Fluor 525

NBP2-48002JF525

Janelia Fluor 549

NBP2-48002JF549

Janelia Fluor 646

NBP2-48002JF646

Janelia Fluor 669

NBP2-48002JF669

mFluor Violet 450 SE

NBP2-48002MFV450

mFluor Violet 500 SE

NBP2-48002MFV500

mFluor Violet 610 SE

NBP2-48002MFV610

PE

NBP2-48002PE

PerCP

NBP2-48002PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-48002

IHC-Prediluted

NBP2-48186

Product Specifications
Applications
Spectra Viewer
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Chimpanzee, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # HSPB1/774

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

Recombinant human HSP27 protein (Uniprot: P04792)

Reactivity Notes

Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken. Others-not known.

Localization

Predominantly cytoplasmic with some nuclear

Specificity

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for HSP27 Antibody (HSPB1/774) [DyLight 550]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NBP2-48002. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 Antibody (HSPB1/774) [DyLight 550]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HSP27 Antibody (HSPB1/774) [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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